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Boric Acid Inhibits Germination and Colonization of Saprolegnia Spores In Vitro and In Vivo

Figure 1

In vitro effect of boric acid on the germination and colonization of Saprolegnia spores.

Effect of boric acid on the germination and colonization of Saprolegnia spores following 48 hours incubation. (a1 and a2) Grade 3: Profuse Saprolegnia hyphal growth on sesame seeds in the non- treated control group (water). (b1 and b2) Grade 3: germinating spores with mycelial growth on the sesame seeds (boric acid <0.2 g/L). (c1 and c2) Grade 2: reduced germination rate and minimal mycelial growth on the sesame seeds (boric acid 0.2–0.4 g/L). (d1 and d2) Grade 1: germinating spores without growing mycelia on the sesame seeds (boric acid 0.5–0.7 g/L). (e1 and e2) Grade 0: no germinating spores, no growing mycelia on the sesame seeds (boric acid ≥0.8 g/L). (f1 and f2) Grade 0: positive control group (bronopol), no spore germination with absence of the growing mycelia on sesame seeds.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0091878.g001