Sampling Strategies and Biodiversity of Influenza A Subtypes in Wild Birds
Figure 3
Subtype richness and sampling effort varies among studies.
(a) Predicted AIV subtype richness (diamonds) with 95% confidence intervals based on the mean Chao2 richness estimator for seven distinct study locations for which the criteria of five sampling events and >5,000 birds tested non-discriminately for AIV subtypes were met (Table? 1). Further, predicted AIV subtype Chao2 richness estimates are displayed from GenBank. (b) The sample size at each study location (blue bar) and minimum sufficient sampling size necessary to capture 75% of total estimated AIV richness (black dot). EGY = Egypt [50], PRT = Portugal [51], MNG = Mongolia (this study), EU = European Union (this study), CAN = Alberta, Canada [34], SWE = Sweden [14], TWN = Taiwan [37], and GB = sequences with subtypes deposited in GenBank (1959–2012).