Early Growth Inhibition Is Followed by Increased Metastatic Disease with Vitamin D (Calcitriol) Treatment in the TRAMP Model of Prostate Cancer
Figure 1
Schematic of prevention studies and safety of vitamin D compounds in TRAMP mice.
(A) Schematic of the time course of chemoprevention studies. All mice were castrated at 12 weeks in the hormone refractory studies. (B–C) Four week-old TRAMP mice were treated with vehicle (control), calcitriol (20 µg/kg) or QW (50 µg/kg) i.p. 3×/week for 14 weeks. (B) Final body weights (g) of control (n = 40), calcitriol (n = 41) and QW (n = 42) mice measured 72 h after last treatment. (C) Serum calcium (mg/dL) levels for control (n = 39), calcitriol (n = 40) and QW (n = 42) mice measured 72 h after last treatment. All data are represented as box plots with medians, and whiskers representing the 5th and 95th percentiles. • (dots), represent points outside the 5th and 95th percentile. All p-values were generated using the Mann-Whitney test.