Establishment and Characterization of an Omasal Epithelial Cell Model Derived from Dairy Calves for the Study of Small Peptide Absorption
Figure 2
Morphology of isolated omasal epithelial cells at different growth periods.
A: Epithelial cell suspensions obtained from newborn calves after enzymatic digestion (×200); B: Most of the omasal epithelial cells adhered to the wall of plastic substratum with small number of fibroblasts after 12 h in culture (×200); C: Cell clusters were observed after 2 d in culture (×100); D: A confluent monolayer formed after approximately one week in culture (×100); E: Purified cultures displayed a cobble-stone-like morphology (×100); F: A confluent monolayer of spindle-shaped, fibroblast-like cells (×100); Fibroblast-like cells are indicated by the arrows.