Imaging Dynamic Molecular Signaling by the Cdc42 GTPase within the Developing CNS
Figure 11
A. All pixels from an image are plotted on a single Polarplot. Averages of fluorescence lifetime in individual pixels cluster around a ‘mean’ lifetime (red arrow). B. The known baseline lifetime, i.e., 2.56 ns, defines 0% FRET percentage on the semicircle (black open circle). A straight line projecting through the ‘mean’ lifetime (black circle) intersects with the semicircle at 100% FRET percentage (grey open circle). The position of the ‘mean’ in respect to the 0% and100% FRET is the estimate of percentage of interaction between the pair of proteins examined.