Comparison of Blood RNA Extraction Methods Used for Gene Expression Profiling in Amyotrophic Lateral Sclerosis
Figure 9
Real-time validation PCR for genes in LL and TEM GC gene lists.
ΔCTs plotted for 6 genes chosen for validation using the LL method of RNA extraction from blood (A). Four out of 6 genes (CDK1, EGR1, SSPN and IL23) could be validated from the Affymetrix microarray studies (C). ΔCTs plotted for 6 genes chosen for validation using the TEMPUS/GC method of RNA extraction from blood (B). Four out of 6 genes (EP400, E2F2, EHBP1 and KAZ) could be validated from the Affymetrix microarray studies (D). Blue bars represent levels of gene expression in ALS patients (n = 6) and red bars the levels in normal control individuals (n = 6).