Reconstruction of the Carotenoid Biosynthetic Pathway of Cronobacter sakazakii BAA894 in Escherichia coli
Figure 4
Zeaxanthin-monoglycoside and zeaxanthin-diglycoside could be synthesized in E. coli when seven C. sakazakii genes were expressed.
A. TLC analysis of carotenoids EC1 and EC2 produced by DH5α/pWSK29-EiZYIBX. B. UV-visible spectra of purified EC1 and EC2. C. ESI/MS analysis of purified EC1. D. ESI/MS analysis of purified EC2. E. HPLC analysis of the hexose hydrolyzed from EC1.