Evaluation of Reference Genes for Accurate Normalization of Gene Expression for Real Time-Quantitative PCR in Pyrus pyrifolia Using Different Tissue Samples and Seasonal Conditions
Figure 4
Normalized expression level of PpCBF2 and PpCBF4.
(A) Normalization by best stable genes for overall samples (HIS_cds, SAND_utr, TIP_utr). (B) By least stable genes for overall samples (bTUB_utr3, ACT_utr2, bTUB_utr2). (C) By best stable genes for FB (bTUB_utr3, HIS_utr2, ANX_utr). (D) By least stable genes for FB (EF1a_cds, GAP_utr2, bTUB_utr2).