Autophagy Adaptor Protein p62/SQSTM1 and Autophagy-Related Gene Atg5 Mediate Autophagosome Formation in Response to Mycobacterium tuberculosis Infection in Dendritic Cells
Figure 3
Maturation of mycobacterial autophagosomes in DC2.4 cells.
(A) Recruitment of p62 to LC3-positive mycobacteria. DC2.4 cells were infected with Alexa Fluor 405-labeled M. tuberculosis (blue) for 24 h and immunostained with anti-LC3 (green) and anti-p62 antibodies (red). (B) The proportion of p62 localization to LC3-positive mycobacteria in DC2.4 cells.
(C) Recruitment of ubiquitin to p62-positive mycobacteria. DC2.4 cells were infected with Alexa Fluor 405-labeled M. tuberculosis (blue) for 24 h and immunostained with anti-p62 (red) and anti-ubiquitin antibodies (green). (D) The proportion of ubiquitin localization to p62-positive mycobacteria in DC2.4 cells. Data represent the mean and SD of three independent experiments.