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The Effect of Hydrophilic Ionic Liquids 1-Ethyl-3-Methylimidazolium Lactate and Choline Lactate on Lipid Vesicle Fusion

Figure 6

TEM imaging of liposomes with ILs and PEG.

After the addition of each [Ch][Lac] and [EMI][Lac] to liposome solutions, and lipids composition and ratio of liposomes were same with FRET and DLS measurements. Samples were put on mesh for EM, and the removal of excess water/sample was carried out. Samples were then set into the TEM. In the case of [Ch][Lac]-liposomes (A), there are various sizes of liposomes between 340−780 nm. In the case of [EMI][Lac]-liposomes (B), liposomes became larger than [Ch][Lac]-liposomes, and the sizes were approximately 1−1.7 μm. Both of the IL-liposomes shapes remained circular.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0085467.g006