Subgenomic Reporter RNA System for Detection of Alphavirus Infection in Mosquitoes
Figure 4
Stably Transformed C6/36 cells express reporter mCherry during SINV infection A)
Brightfield, GFP, mCherry, and overlay images are shown from SINV-GFP infected C636 cells that are pBG446 transiently transfected (top) or pBG461 stably selected cells (bottom). Infected with an MOI of 10. Images collected at 48hours post infection. B) Average mCherry fluorescence is significantly higher in pBG461 stably transformed C6/36 cells than transiently transfected cells. C) Averages of total red fluorescence was calculated as a percent of total green fluorescence and is displayed for pBG446 transfected, pBG461stably selected, or C636 mock cells.