ADAM10 Negatively Regulates Neuronal Differentiation during Spinal Cord Development
Figure 4
Downregulation of ADAM10 inhibits cell proliferation in the ventral spinal cord.
After electroporation at E4, transverse sections were used for detection at E6. The transfected cells are marked by green fluorescence, and the control morpholinos (Fluo-m) and the untrasfected side (left side) serve as controls. The immune reactive cells are stained by red. Cell nuclei are labeled by DAPI (blue). (A–X) Immunohistochemistry was performed in adjacent sections with antibodies against BrdU (A–D), Pax6 (E–L), NKx6.1 (M–P), Pax7 (Q–T), NKx2.2 (U–X), respectively. Arrows in (A–D) indicate decrease of BrdU-labeled cells; Arrows in (I–O) indicate the immune negative region within the transfected side. (Y) Quantitative data of the cell number in different domains normalized by the number of the control side, which is set to be 1. All data are presented as mean ± SEM from at least 3 independent experiments (**p<0.01, and ***p<0.001 compared to the control). Scale bar, 200 µm in (I) for (A–W).