An Inducible, Ligand-Independent Receptor Activator of NF-κB Gene to Control Osteoclast Differentiation from Monocytic Precursors
Figure 4
CID induced osteoclasts formed on native mineralized substrates.
(A) TRAP-positive osteoclasts on human dentin slices. Dentin slices created from human teeth were seeded with RAW264.7+iRANK cells and cultured in the presence of AP20187 for 9 days. The slides were fixed and stained for TRAP and multinucleated TRAP-positive cells were shown in purple (arrow) (scale bar = 100 µm). (B) Confocal micrograph of multinucleated osteoclasts on mouse calvarial disc. Calvarial discs were seeded with RAW264.7+iRANK cells and osteoclast formation was induced by the addition of AP20187. Cells were imaged by confocal microscope; blue fluorescence indicates nuclei by Hoechst stain, while green fluorescence indicates GFP expression in RAW264.7+iRANK cells (scale bar = 50 µm).