Functional Analysis LRP6 Novel Mutations in Patients with Coronary Artery Disease
Figure 4
Mutant LRP6 reduce ability to enhance cellular proliferation and migration.
A Expression of wild-type LRP6 and mutant LRP6 was no difference in the total expression levels in HUVECs cells. B In CCK8 assay, overexpression of wild type LRP6 stimulated HUVECs proliferation, whereas overexpression of LRP6 mutant stimulated cell proliferation to a minor extent. C and D Transwell chamber assay shows that the ability of enhancing HUVECs cells migration was reduced in mutant group compared with wild type. WT, wild-type. *P<0.05 vs. PC 3.1, #P<0.05 vs. WT group.