Characterization of Antigen-Specific B Cells Using Nominal Antigen-Coated Flow-Beads
Figure 4
Separation of B cells interacting with nominal antigen and unbound B cells.
Purified B cells were incubated with single HLA class I coated beads (A) or MOG1–125 coated beads (B) before being subjected to cell separation using an ARIA FACS-sorter (A) or magnet based purification (B). Frequency of B cells interacting with nominal antigens is shown before purification and in the positive and in the negative fraction. One representative out of three experiments with cells from different donors is shown.