Molecular and Biochemical Analyses of CbCel9A/Cel48A, a Highly Secreted Multi-Modular Cellulase by Caldicellulosiruptor bescii during Growth on Crystalline Cellulose
Figure 7
Effect of cellulose hydrolysis by four endoglucanases in the presence of either CbCel9A/Cel48A WT or the TM2 mutant.
4EGs: a mixture of four recombinant endoglucanses from C. bescii (CbMan5B/Cel44A-TM2, CbCel9B/Man5A-TM1, CbMan5C/Cel5A-TM2, and CbCel5B-TM1). The reactions were carried out in a citrate buffer (pH 5.5) at 70°C for 16 h, by incubating different concentrations (0, 0.5, 1.0, and 2.0 µM) of WT or TM2 with 5 mg ml-1 of Avicel, 5 mg ml-1 of PASC, or 10 mg ml-1 of Miscanthus, in the presence of the four endoglucanases (4EGs, each at 0.5 µM).