Molecular and Biochemical Analyses of CbCel9A/Cel48A, a Highly Secreted Multi-Modular Cellulase by Caldicellulosiruptor bescii during Growth on Crystalline Cellulose
Figure 4
Time course hydrolysis of cellotetraose (G4) and cellopentaose (G5) by CbCel9A/Cel48A.
The reactions were performed by incubating 0µM (for the control) or 0.2 µM of each enzyme with 2.5 mg ml-1 of cellotetraose (G4) or cellopentaose (G5) in a citrate buffer (pH 6.0) at 75°C for 24 h. Samples were taken at different time points and analyzed using an HPLC method.