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Gene Expression and Functional Annotation of the Human and Mouse Choroid Plexus Epithelium

Figure 7

Confirmation of microarray results by sqRT-PCR.

Beta actin, a household gene, was used to normalize gene expression in the human and mouse CPE. With semi quantitative RT-PCR (sqRT-PCR), we could confirm our microarray results, namely two genes that were substantially higher expressed in human CPE compared to mouse CPE (FBP2 and NELL2), five genes that were highly expressed in both human and mouse CPE (ATP1B1, IGFBP7, NPC2, RPL13, and SLC13A4) and five genes that were substantially higher expressed in mouse CPE compared to human CPE (ACE, LBP, PON1, SLC7A10, and TRIM3). For all genes, positive controls were positive. For example, the primer set for human ACE did showed PCR product in the positive control sample of placenta cDNA, and when we performed PCR in human CPE sample, we found no PCR product for ACE, indicating that there was hardly any RNA of ACE in the human CPE. This was in correspondence of the microarray data. Black bars indicate human CPE expression and grey bars mouse CPE expression. Standard deviations were calculated and indicated in the bars.

Figure 7

doi: https://doi.org/10.1371/journal.pone.0083345.g007