Role of Electrical Activity in Horizontal Axon Growth in the Developing Cortex: A Time-Lapse Study Using Optogenetic Stimulation
Figure 2
Electrophysiological responses of ChR2-expressing cortical neurons to photostimulation.
A and B, ChR2-expressing cells among layer 2/3 neurons were subjected to patch-clamp recording at 4 DIV. Interrupted lines in A indicate the presumed layer 2/3 borders. The white line in B indicates the pial surface. Bar, 200 µm. C, Each flash with 475-nm wavelength light (50-msec duration in the upper trace and 200-msec duration in the lower trace) elicited depolarization and a single action potential.