Reciprocal Regulation of Development of Neutrophil-Dendritic Cell Hybrids in Mice by IL-4 and Interferon-Gamma
Figure 4
Opposing impacts of IL-4 and IFNγ on GM-CSF-induced hybrid cell development.
BM cells were cultured in 6-well plates for 6 days in the presence of 10 ng/ml GM-CSF plus IL-4 (A) or IFNγ (B) added at the indicated concentrations. The samples were harvested and then examined for surface expression of CD11c, MHC II, and Ly6G. The data shown are the absolute numbers (closed circles) and the percentages (open circles) of CD11c+/MHC II+/Ly6G+ neutrophil-DC hybrids (means ± SD from triplicate samples). Asterisks indicate statistically significant differences compared with the control cultures without added GM-CSF (*P < 0.05, **P < 0.01, ***P < 0.001). Data are representative of at least four independent experiments.