Reciprocal Regulation of Development of Neutrophil-Dendritic Cell Hybrids in Mice by IL-4 and Interferon-Gamma
Figure 1
Differential impacts of 61 cytokines on hybrid cell development in crude BM cultures.
BM cells freshly procured from adult C57BL/6 mice were cultured in 96-well plates for 6 days in the presence of each of 61 different cytokines (10 ng/ml). The samples were examined in a semi-automated fashion for surface expression of CD11c, MHC II, and Ly6G. (A) Each dot represents the percentage of CD11c-/MHC II-/Ly6G+ neutrophils, CD11c+/MHC II+/Ly6G+ hybrids, or CD11c+/MHC II+/Ly6G− conventional DCs emerging in BM culture supplemented with a given cytokine. The baseline levels (means ± 3SD) observed in vehicle alone control cultures without added cytokines are shown on the left columns. “Hit” cytokines that increased or decreased the frequencies above or below the baseline levels are indicated in red. The data shown are representative of three independent screening experiments with similar results. (B) The hit cytokines were tested in triplicates at 10 ng/ml. The data shown are the means ± SD of the percentages of neutrophils, hybrids, and conventional DCs. Asterisks indicate statistically significant differences compared with the control cultures without added cytokines (*P < 0.05, **P < 0.01, ***P < 0.001).