Production of Hybrid-IgG/IgA Plantibodies with Neutralizing Activity against Shiga Toxin 1
Figure 3
Quantification of assembled hybrid-IgG/IgA on sandwich ELISA.
A. Hybrid-IgG/IgA in extracts of transgenic A. thaliana leaves, which contained the indicated amounts of total soluble protein (TSP), was captured with immobilized goat anti-mouse κ and then detected with HRP-goat anti-mouse IgA (α chain-specific). The samples were from dimer Tg plants (closed circles) or wild-type plants (closed triangles). B. Standard curve for quantification of IgA. IgA molecules that had been captured by the immobilized goat anti-mouse κ chain were detected with HRP-goat anti-mouse IgA on ELISA. Various concentrations of purified mouse myeloma protein TEPC 15 were used to generate a standard curve. Data are expressed as means ± SD of triplicate determinations. Error bars underneath the symbols are not visible. The results are representative of three experiments.