Loss of Leucine-Rich Repeat Kinase 2 (LRRK2) in Rats Leads to Progressive Abnormal Phenotypes in Peripheral Organs
Figure 3
2-month old LRRK2 KO rat (Cortex, kidney).
A: Hematoxylin and Eosin – slight increase in size and variability of hyaline droplets in proximal tubular epithelium (open arrow); B: Lipofuscin (AFIP method) – Lipofuscin positive material not observed; C: Chromotrope Aniline Blue – increased size, number and variability of hyaline droplets in proximal tubular epithelium (open arrows). Hyaline droplet variability was more easily identified using CAB staining when compared to H&E stains; D: LAMP-1 – increased cytoplasmic staining intensity in proximal tubular epithelium; E: LAMP-2 – increased globular cytoplasmic staining (solid arrows) with apical cytoplasmic staining- persisting in proximal tubular epithelium; F: NAGLU – increased globular staining in proximal tubular epithelium (solid arrow). Scale bar = 0.5 mm