Anti-Tumoral Effect of the Mitochondrial Target Domain of Noxa Delivered by an Engineered Salmonella typhimurium
Figure 5
Anti-tumoral effects of Salmonellae carrying either pLYSPBAD::DS4.3-MTD or pLYS, Three days after the bacterial treatment, L-arabinose (60 mg) was intraperitoneally injected daily (n = 3 per group).
(A) Changes in tumor size after the bacterial treatment. Data represent mean ± S.D., and asterisks (*) indicate a significant difference compared between each groups with two-way ANOVA (***, P<0.0001) (B) A representative morphological change of the implanted CT26 tumor after the bacterial treatment. (C) Immuno-fluorescence examination of CT26 tumor tissue after bacterial treatment. Frozen section was obtained from the tumor tissue and stained with Noxa specific antibody (SantaCruz, sc-30209). The boxes on left show nucleus with DAPI in blue, Noxa MTD with specific antibody in green, and cytosol with TexasRed Phalloidin in red, in order from the top. The boxes on the right show merged images. (D) Histopathological examination of CT26 tumor tissue after the bacterial treatment. Tumor tissues were sliced and stained with hematoxylin-eosin (HE). Purple area represents the region with healthy cells and whitish pink the region with anucleus dead cells, the necrotic region.