A RhoC Biosensor Reveals Differences in the Activation Kinetics of RhoA and RhoC in Migrating Cells
Figure 3
Correlation of RhoA/C activation and cell edge dynamics.
(A) Top: 2.5 µm width, 0.9 µm depth sampling windows (yellow) are placed at fixed distances from the edge of a MEF expressing the RhoC biosensor. (B) Correlation of each biosensor and cell edge velocity as a function of time and distance from the edge. Inset legend indicates color code for spatial zones. RhoA: n=16 cells, 993 windows, RhoC: n=16 cells, 869 windows. Correlation coefficients (C) and time shifts (D) for RhoA (open) and RhoC (solid) at different distances from the edge. Error bars represent 95% confidence intervals, estimated by bootstrap analysis of variation in the correlation functions.