Use of the γ-H2AX Assay to Investigate DNA Repair Dynamics Following Multiple Radiation Exposures
Figure 3
γ-H2AX foci kinetics following split multiple radiation exposures.
Number of γ-H2AX foci per cell after exposure to 1 Gy at time 0 plus 1 Gy delivered20 min later (panel A), 1 hour later (panel B), 2 hours later (panel C), 5 hours later (panel D) and 12 hours later (panel E) using 225 kVp X-rays. In Panel F representative pictures of cells in different exposures scenario are presented, i.e. cells fixed after 30 minutes in the cases of 20 minutes split dose exposure (Figure 1), 1 hour split dose exposure (Figure 2), 2 hours split dose exposure (Figure 3) and 5 hours split dose exposure (Figure 4). Error bars represent one standard error of the mean of 3 independent experiments. Lines are guides for the eyes.