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An Ultrasensitive High Throughput Screen for DNA Methyltransferase 1-Targeted Molecular Probes

Figure 5

Dose-response analysis of the identified direct DNMT1 inhibitors.

IC50 values were determined at 20 nM oligonucleotide 8006 and 10 µM SAM for each inhibitor (13– black •; 22– red ▪; 24– blue ▴; 26– green ▾; 29– orange •; 33– purple ▪; 36– pink ▴, 40– grey ▾, 51– navy •). Inhibitor concentration was varied from 0.1–10 µM. The percent activity was determined by comparing to an uninhibited DMSO-containing control. The data were fit using a Hill slope of -1 to obtain IC50 values (Table 1).

Figure 5

doi: https://doi.org/10.1371/journal.pone.0078752.g005