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Comparison of RNA-Seq and Microarray in Transcriptome Profiling of Activated T Cells

Figure 7

Comparison of RNA-Seq and Affymetrix microarray in detection of genes expressed at high levels.

β-actin (ACTB) is expressed at high levels in all conditions as measured by both RNA-Seq and microarray. Microarray detected similar levels of ACTB between resting and activated T cells whereas RNA-Seq showed a 2 to 4-fold increase in activated T cells. ACTB has been reported to be 5.3-fold up-regulated in in-vitro stimulated lymphocytes measured by quantitative polymerase chain reactions [26].

Figure 7

doi: https://doi.org/10.1371/journal.pone.0078644.g007