Slow Proton Transfer Coupled to Unfolding Explains the Puzzling Results of Single-Molecule Experiments on BBL, a Paradigmatic Downhill Folding Protein
Figure 6
T-jump folding-unfolding relaxation kinetics of BBL at various final temperatures measured at pH 8 and near the chemical denaturation midpoint.
The left panels show the experimental decays measured in logarithmic time and fitted to double (green) and single (red) exponential functions. The right panels show the same experimental data plotted in linear time and fitted to a single-exponential function.