Expression and Antimicrobial Function of Beta-Defensin 1 in the Lower Urinary Tract
Figure 2
Effect of Defb1 deficiency on UPEC burden.
(A) RT-PCR confirms absent expression of Defb1 mRNA in kidneys of Defb1-/- mice (-/-, n=2), versus presence of the predict PCR product in wild type kidneys (+/+). Gapdh RT-PCR is included as a loading control. (B) Bacterial burden in bladders and kidneys of wild type (WT) versus Defb1-/- (knockout, KO) mice at indicated timepoints following UPEC inoculation. Compiled data from at least 2 separate experiments in 8 mice are shown. The horizontal lines indicate geometric means. The dashed horizontal line indicates the lower limit of detection. There was no significant difference between geometric means at any time following infection in bladders or kidneys (Mann-Whitney Test, p > 0.05). (C) Bacterial burden in bladders and kidneys of wild type (WT) versus Defb1-/- (knockout, KO) mice at 24 hpi following inoculation with 5x105 CFU UTI89.