Combining Different mRNA Capture Methods to Analyze the Transcriptome: Analysis of the Xenopus laevis Transcriptome
Figure 4
Overview of combined X. laevis transcriptome.
A. Sequences from each assembler:library pair were combined. Transcripts from this combined library were sequentially aligned to X. tropicalis (Xt) ENSEMBL transcripts, X. tropicalis Genscan transcripts, and human Uniprot proteins using BLASTX. The number of transcripts that matched to each annotation source and the number of annotated transcripts that were matched are indicated. B. UCSC genome browser view that demonstrates clustering of transcript fragments by using the annotated ENSEMBL transcript as a scaffold. C. UCSC genome browser view of transcript clustering of transcripts that match to a GENSCAN gene prediction. D. Example of several transcripts that match to an annotated human protein while there are no predicted X. tropicalis transcripts.