Ewing Sarcoma Cells Secrete EWS/Fli-1 Fusion mRNA via Microvesicles
Figure 5
Detection of EWS/Fli-1 mRNAs in MVs isolated from blood samples taken from ES cell/xenografted mice.
The method of isolation of MVs from the blood samples was described in Materials and Methods. Confirmation of the EWS/Fli-1 mRNAs as the quantitative PCR products was performed. Electrophoretograms of the qRT-PCR products are shown.
Among the 10 TC135 tumor-bearing mice, 4 of them were positive for the fusion mRNA. In the case of the 7 A673 tumor-bearing mice, 3 of them were fusion mRNA positive.
The positive control from TC135 and A673 cells (P) and non-template control (NC) are also shown.