Generation of Breast Cancer Stem Cells by Steroid Hormones in Irradiated Human Mammary Cell Lines
Figure 2
Proportion of CSCs after irradiation and steroid hormone treatment.
The percentages of ALDH+ T47D (A), MCF7 (B) and MCF10A (C) cells were evaluated by flow cytometry three days after irradiation. Hormonal treatment was performed two days before irradiation and every day afterwards. Pg: progesterone, Mif: mifespristone, E2: estrogen, Inh: PD173074. Results are representative of at least three independent experiments. Error bars represent standard deviation. Asterisks denote significant differences (t-test, *p<0.05, **p<0.01).