Reactive Oxygen Species Are Required for 5-HT-Induced Transactivation of Neuronal Platelet-Derived Growth Factor and TrkB Receptors, but Not for ERK1/2 Activation
Figure 2
H2O2 concentrations sufficient for inducing PDGFβ receptor phosphorylation do not result in cell death.
SH-SY5Y cells were treated with 0, 0.1, 1, 10, 100, or 1000 µM H2O2 for (A) 30 min, or (B) overnight. Following treatment with MTT reagents and lysis, cell viability was measured and compared to control (VEH) values. (Data are representative of 4 independent experiments. * = p < 0.05 compared to vehicle-treated cells, one-way ANOVA, Tukey post-test).