Recombinant AAV-Mediated BEST1 Transfer to the Retinal Pigment Epithelium: Analysis of Serotype-Dependent Retinal Effects
Figure 1
Recombinant AAV-mediated GFP expression in the canine eyes by fundus autofluorescence imaging.
A representative summary of findings from rAAV2/2-hVMD2-GFP injected canine eyes. A normalized autofluorescence mode was used to obtain a baseline photograph for the native eye before vector administration (A). GFP fluorescence was first detectable at 2 weeks p.i. (B, arrowheads) and the outline of the injection area corresponded to the subretinal bleb documented immediately after vector administration (A, inset). An evident increase in GFP expression was observed at 4 to 6 weeks p.i. (C, D), when the peak of rAAV vector expression is expected. The boundaries of the autofluorescent areas (arrowheads), as well as retinotomy scar (arrows) remained unchanged throughout the observation period. High-resolution images were captured in AF mode at 55°; p.i.: post injection.