Drug-Induced Reactivation of Apoptosis Abrogates HIV-1 Infection
Figure 3
Effects of ciclopirox on cellular and retroviral proteins in H9 cells.
A, B. Bcl-2 reactivity of cells was quantified by flow cytometry after 24 hr of treatment with CPX. H9-HIV are shown in red, H9 in blue. A: Cell counts over the fluorescence intensity spectrum for Bcl-2 reactivity in cells treated with 30 µM CPX. B: CPX concentration dependence of Bcl-2 reactivity expressed as the geometric mean of fluorescence (mean ± SEM). C. Response of proteins in H9-HIV cells to 30 µM CPX after exposure for 24 hr (hatched bars) and 48 hr (filled bars). Retroviral and cellular proteins were labeled immunocytochemically and quantified in the same sample by flow cytometry. Data are presented as the geometric mean of fluorescence, normalized to time-identical infected untreated controls (100% values at 24/48 hr: p24, 36.2/38.2; Tat, 168.1/141.6; Rev, 7.8/6.4; Vpr, 1.7/1.7; activated caspase-3, 1.3/1.3). P values for deviation from respective controls are indicated: * = 0.02; ** ≤ 0.004; *** ≤ 0.0004.