Drug-Induced Reactivation of Apoptosis Abrogates HIV-1 Infection
Figure 2
Ciclopirox increases apoptosis preferentially in HIV-infected H9 cells.
A. Increased formation of the caspase-3–fragmented 89-kDa form of PARP in H9-HIV (red) and uninfected H9 (blue) after 24 hr of treatment with 30 µM CPX. Results (mean ± SEM) are presented as the fold-increase in PARP fragment-positive cells relative to untreated cells. B, C. Cell counts over the fluorescence intensity spectrum for 89-kDa PARP reactivity, quantified by flow cytometric single cell analysis after 24 hr (B) and 48 hr (C) of treatment with 30 µM CPX. Percentages of frag-PARP–positive H9-HIV (red) and uninfected H9 (blue) are calculated.