Calcium Sensing Receptor Expression in Ovine Amniotic Fluid Mesenchymal Stem Cells and the Potential Role of R-568 during Osteogenic Differentiation
Figure 4
Effect of calcimimetics on ALP activity.
ALP activity, detected at day 7, on oAFMSCs treated with calcimimetics pre-incubated (30 minutes) or not with Calhex-231 (1 µM, *p<0.0001 2.5 mM CaCl2 vs Basal 1.8 mM CaCl2 and R-568 vs Basal 1.8 mM CaCl2; §p<0.01 R-568+2.5 mM CaCl2 vs S-568+2.5 mM CaCl2; **p<0.001 R-568+2.5 mM CaCl2 vs 2.5 mM CaCl2 and R-568+Calhex+2.5 mM CaCl2 vs R-568+2.5 mM CaCl2; #p<0.002 R-568+Calhex vs R-568+1.8 mM CaCl2). ALP activities were calculated with p-nitrophenol standard and normalized per mg of proteins.