Differential Effect of Actomyosin Relaxation on the Dynamic Properties of Focal Adhesion Proteins
Figure 3
Differential dissociation rates of adhesome components in cells treated with Y-27632.
(A) FA lifespan (expressed as the relative number of surviving FAs) and average normalized fluorescence intensity for each protein, in peripheral and central FAs separately, as tracked by time-lapse video microsopy for a hundred individual FAs. τ values were calculated by fitting the individual decay profiles to a single-exponential function. Note that the order of exit of the different molecules is the same in all graphs, with zyxin and VASP as the first to leave and kindlin 2 as the last. (B) Based on the same dataset shown in A, the differential effect of Y-27632 on the lifespan of the FA proteins tested, in peripheral FAs, is shown for each protein, separately. FA lifespan for each protein is expressed as the percentage of total FA number over time. Untreated cells are shown as white columns; cells treated with Y27632 as black columns.