Pharmacological Inhibition of polysialyltransferase ST8SiaII Modulates Tumour Cell Migration
Figure 4
Effect of ST8SiaII inhibition on recovery of polySia expression following removal by endoN.
C6-STX cells (left) and SH-SY5Y cells (right) immunolabelled with anti-polySia antibody (mAb 735) followed by incubation with TRITC-conjugated secondary antibody [PolySia orange; nuclei stained blue with DAPI]. (a) Negative control (absence of mAb 735); (b) Positive control (absence of endoN/CMP treatment) (c) Removal of polySia with EndoN (0.3 µg/mL); (d) PolySia recovery following 6 h incubation in absence of CMP; (e) PolySia recovery following 6 h incubation with CMP at 0.5 mM; (f) PolySia recovery following 6 h incubation with CMP at 5 mM. CMP clearly prevents the recovery of polySia on the cell surface following biological removal at 5 mM. Scale bar = 50 µm.