Highly Pathogenic Avian Influenza Virus Nucleoprotein Interacts with TREX Complex Adaptor Protein Aly/REF
Figure 5
Interaction between NP of pandemic Influenza A/Malaysia/854/2009(H1N1) virus with human THO complex 4 (THOC4) or Aly/REF (Experiment III).
Mammalian two hybrid assay was conducted in 24-well plate. HEK293T cells were transfected with respective plasmids (Table 2) in triplicates, incubated at 37°C, 5% CO2, for 48 hours. Culture media were harvested and tested for SEAP activity using GreatEscAPe™ SEAP Chemiluminescence Detection Kit (Clontech). The values reported are averages from 3 independent transfections (±S.D.). SEAP activity directly reflects the interaction between pandemic H1N1 NP and human THO complex 4 (THOC4). Results show that NP- human Aly/REF interaction was also relatively strong and statistically comparable (p-value >0.05) to when compared to the positive control pM3-VP16 and other 2 experiments conducted with different subtypes of Influenza.