Swiprosin-1 Is a Novel Actin Bundling Protein That Regulates Cell Spreading and Migration
Figure 4
Swiprosin-1 induces actin bundling in vitro.
(A) F-actin (2 µM) was incubated with various concentrations of His_Swip-1 (0.25–4 µM) for 30 min. The samples were assessed for actin-bundling activity as described in the Experimental Procedures. The percentage of total actin in the pellet was quantified (bottom). S, supernatant; P, pellet. (B and C) Electron micrographs of actin filaments (2 µM) after incubation with or without His_Swip-1 (4 µM) (B) or GST_Swip-1 (4 µM) (C).