Behavioral and Molecular Analysis of Nicotine-Conditioned Place Preference in Zebrafish
Figure 4
Baseline (pretest) and test values of behavioral parameters in the non-preferred compartment in nicotine-CPP.
CPP was performed by using 15 mg/L of nicotine during conditioning and the time course examined was of 5 min following 5 min habituation during the pretest and test sessions. Panel a) shows the time spent in the white compartment, and b) the number of transitions to the white compartment. c) This graph shows the average entry duration to the white compartment. Data are presented as mean ± SEM. Control: saline n = 15, Nic-unpaired n = 12, and Nic-paired n = 21. *p<0.05 and **p<0.01 between pretest and test and #p<0.05, ##p<0.01 and ###p<0.001 between controls (saline and Nic-unpaired) and Nic-paired. Control: saline; Nic-unpaired: counterbalanced nicotine treatment and Nic-paired: nicotine treatment associated to the white compartment.