The Histidine-Phosphocarrier Protein of the Phosphoenolpyruvate: Sugar Phosphotransferase System of Bacillus sphaericus Self-Associates
Figure 4
Thermal and chemical denaturations of HPrbs.
(A) Thermal denaturations followed by the changes in ellipticity at 222 nm (left side, blank circles) and in the intrinsic fluorescence emission at 305 nm, after excitation at 278 nm (right side, filled blue circles). (B) Urea-denaturations followed by the changes in ellipticity at 222 nm (left side, blank circles) and in the intrinsic fluorescence emission at 305 nm (similar results were obtained at 315 and 330 nm), after excitation at 278 nm (right side, blue filled circles).