P45 Forms a Complex with FADD and Promotes Neuronal Cell Survival Following Spinal Cord Injury
Figure 4
TUNEL assay shows that Thy1-p45 mice have decreased cell death following stab wound injury compared to their WT littermates.
One (A, B) or 3 days (C, D) after stab wound injuries on the right side of T13 spinal cord, Thy1-p45 mice (B, D) had fewer TUNEL+ cells compared to their WT littermates (A, C) on the injured side of the spinal cord. Serving as an internal control, there were almost no TUNEL+ cells on the contralateral uninjured side of the spinal cord in every section. The TUNEL+ cells co-localized with Hoechest 33342 nuclei staining and showed the typical features of cell death (DNA fragmentation and condensation) in the magnified view of injured spinal cord from both Thy1-p45 mice and their WT littermates (E–F). Scale bars: 200 µm (A–D), 20 µm (E, F).