MHC Class I Antigen Presentation of DRiP-Derived Peptides from a Model Antigen Is Not Dependent on the AAA ATPase p97
Figure 5
Inhibition of p97 does not diminish over all levels of cell-surface MHC class I.
A. EL4 cells were transfected with p97 constructs and analyzed 24 hours later for cell-surface Kb expression by FACS by gating onThy1.1 cells. Cells were either left untreated (white bars) or washed in citric acid buffer (black bars) and allowed to recover for five hours before analysis. The staining was done in triplicate. B. EL4 cells were treated with indicated levels of DBeQ for 5 hours and analyzed by FACS for cell-surface Kb expression. No consistent statistical difference at any concentration of DBeQ occurred between experiments, though MG132 did statistically diminish Kb levels (p<0.05). In both experiments, EL4 cells treated with 10 µM MG132 for 5 hours was included as a positive control for inhibiting antigen presentation and diminishing cell-surface Kb.