Specialized Yeast Ribosomes: A Customized Tool for Selective mRNA Translation
Figure 2
Luciferase expression profiles in 124 yeast strains each with a different individual specialized ribosome variant.
The luciferase readouts of each of the four individual FF-based reporters, co-expressed with the REN reporter, are shown as follows: (A) FF/REN and FFPTC/REN; (B) LA3FF/REN and LA3PTCFF/REN. The luciferase readouts were monitored in all 124 yeast strains each with a different individual specialized ribosome variant, and sorted according to expression strength: lowest levels to the left, highest on the right. The REN profiles are shown in yellow and the individual FF-based reporters are shown in green. For each reporter spectrum the luciferase activities for the wild type parent strain are indicated as a red dot, the grand mean of the given spectrum of assays as a black dot and standard deviation of the grand mean as black triangles.