IgE Reactivity of Blue Swimmer Crab (Portunus pelagicus) Tropomyosin, Por p 1, and Other Allergens; Cross-Reactivity with Black Tiger Prawn and Effects of Heating
Figure 5
Characterization of recombinant blue swimmer crab tropomyosin, rPor p 1.
A. 4–12% SDS-PAGE of recombinant blue swimmer crab TM, rPor p 1 stained with Coomassie brilliant blue. M, MW markers. B. Sera from 12 shellfish-allergic subjects (5–24) and one non-atopic (NA) subject were tested for IgE reactivity to rPor p 1 using direct IgE immunoblotting.