Tissue-Specific 5′ Heterogeneity of PPARα Transcripts and Their Differential Regulation by Leptin
Figure 1
Tissue specific 5′ heterogeneity of PPARα transcripts.
A) 5′ RLM RACE PCR indicates 2 major PPARα transcripts in liver and one in adipose. M, DNA ladder; NTC, no template control; +C, RACE positive control; -C, negative control; -C, -TAP control; liver, liver cDNA; adipose, adipose cDNA; M, DNA ladder. B) A schematic diagram showing the genomic organisation of the rat PPARα gene. Location of PPARα 5′UTR exons on the genomic sequence are shown (non-coding exons, black; coding exons, white and the updated exons, grey). All exon positions are indicated relative to the Ensembl transcription start site. Ensembl exon 3 which contains the translation ATG start codon is present in all transcripts. C) Diagram showing the adipose specific transcript (P1) and liver specific transcripts (P2 and P3). D) Comparison of the Rat PPARα 5′UTR with the 5′UTR of the human and mouse PPARα genes. Non-coding 5′UTR exons are shown in black, coding exons in white and newly identified non coding exons in grey.