MeCP2 Regulates the Synaptic Expression of a Dysbindin-BLOC-1 Network Component in Mouse Brain and Human Induced Pluripotent Stem Cell-Derived Neurons
Figure 7
Mecp2 and BLOC-1 Deficiency Affect BDNF Immunoreactivity in the Dentate Gyrus.
Images depict indirect quantitative immunofluorescence confocal microscopy of BDNF and the synaptic vesicle marker synaptophysin in the dentate gyrus from Mecp2tm1.1Jae/y, the BLOC-1-null mice Bloc1s8sdy/sdy, and Bloc1s6pa/pa (A–B). BDNF immunorreactivity was abrogated by preincubation of antibodies with recombinant human BDNF (rhBDNF, A–B). Box plots in C depict the quantitation of BDNF immunoreactivity normalized to synaptophysin. P values were obtained by One Way Anova with Dunnett’s Multiple comparisons correction, n = 4 independent stainings from 2 animals per genotype. D, BDNF transcripts from symptomatic adult mice hippocampi were analyzed by qRT-PCR. Box plot depicts relative mRNA content for wild type (Mecp2+/y, Blue), Mecp2 mutant tissue (Mecp2tm1.1Jae/y, Red), as well as the BLOC-1-null mice Bloc1s8sdy/sdy, and Bloc1s6pa/pa. P values were obtained by One Way Anova with Dunnett’s Multiple comparisons correction, n = 4 animals per genotype.